By David K. Gardner, Michelle Lane, Andrew J. Watson
This booklet pulls jointly the whole diversity of mobile tradition, biochemical, microscopic, and genetic recommendations to review the early mammalian embryo. formerly, there hasn't ever been this type of accomplished compendium, notwithstanding there were extra centred books of protocol, similar to Manipulating the Mouse Embryo, from chilly Spring Harbor. This booklet is meant to entice all constituencies, from uncomplicated experimental technology to scientific and animal technology functions.
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Additional resources for A Laboratory Guide to the Mammalian Embryo
Follicles are selected for culture on the basis of size and morphological characteristics. The oocyte needs to be centrally located surrounded by tightly layered granulosa cells with no obvious gaps between them. To maintain intact follicles that will grow well over the culture period, a small clump of thecal-interstitial tissue needs to be attached to the basement membrane. 4A), but within a day or two of culture, thecal cells from the very edge die, and a smooth, even layer of theca remains round the follicle.
And Day, B. N. (2000). Theriogenology, 54, 787. 33. Goff, A. , and Miron, P. (2001). Reprod. Domest. , 36, 19. 34. , and Cognie, Y. (2001). Theriogenology, 54, 209. 35. Ehrmann, R. , and Gey, G. O. (1956). J. Natl. , 16, 1375. 36. , and Mauchamp, J. (1981). J. , 91, 157. 37. , and Kato, S. (1994). J. Reprod. , 100, 333. 38. , Emery, B. , and Carrell, D. T. (2001). Biol. , 64, 375. 39. , Mobberley, M. , Moore, P. , Margara, R. , and Winston, R. M. (1997). Fertil. , 68, 682. 40. Telfer, E. , Binnie, J.
Best results from superovulation protocols are achieved by using prepubertal females (3–5 weeks old). Two hormones are administered by intraperitoneal injection 48 h apart. The first injection of pregnant mare’s serum gonadotrophin (PMSG) provides FSH that recruits follicles for ovulation. 2. 005g/l For protocols on how to prepare media, see instructions in chapter 3. 3. 001 followed 48 h later by an injection of human choronic gonadotrophin (hCG), which mimics the LH surge and results in ovulation.
A Laboratory Guide to the Mammalian Embryo by David K. Gardner, Michelle Lane, Andrew J. Watson