Get A Laboratory Guide to the Mammalian Embryo PDF

By David K. Gardner, Michelle Lane, Andrew J. Watson

ISBN-10: 0195142268

ISBN-13: 9780195142266

This booklet pulls jointly the whole diversity of mobile tradition, biochemical, microscopic, and genetic recommendations to review the early mammalian embryo. formerly, there hasn't ever been this type of accomplished compendium, notwithstanding there were extra centred books of protocol, similar to Manipulating the Mouse Embryo, from chilly Spring Harbor. This booklet is meant to entice all constituencies, from uncomplicated experimental technology to scientific and animal technology functions.

Show description

Read Online or Download A Laboratory Guide to the Mammalian Embryo PDF

Similar mammals books

Roger Eston, Thomas Reilly, Tom Reilly's Kinanthropometry and Exercise Physiology Laboratory Manual: PDF

Kinanthropometry is the examine of human physique dimension, form and shape and the way these features relate to human stream and wearing functionality. during this totally up to date and revised variation of the vintage consultant to kinanthropometric idea and perform, top foreign game and workout scientists provide a transparent and entire advent to crucial ideas and strategies.

Download e-book for iPad: Mark of the Grizzly: True Stories of Recent Bear Attacks and by Scott McMillion

Needs to learn for a person drawn to those magnificant creatures - choked with the genuine tales of modern endure assaults.

Read e-book online Squirrels: A Wildlife Handbook (Johnson Nature Series) PDF

A mixture of illustrated box consultant, truth e-book, and folklore assortment, "Squirrels" is a brand new type of natural world publication. This concise, illustrated guide offers a correct, informative portrait of squirrels of their traditional habitat, besides an exam in their courting to guy. so much americans stay in towns.

Download e-book for kindle: A Text-Book of the History of Painting (Illustrated Edition) by John C. van Dyke

John Charles van Dyke (1856-1932) used to be an American paintings historian and critic. He used to be born at New Brunswick, N. J. , studied at Columbia, and for a few years in Europe. together with his ebook chronicling the heritage of portray from cave work to the trendy period. totally illustrated.

Additional resources for A Laboratory Guide to the Mammalian Embryo

Example text

Follicles are selected for culture on the basis of size and morphological characteristics. The oocyte needs to be centrally located surrounded by tightly layered granulosa cells with no obvious gaps between them. To maintain intact follicles that will grow well over the culture period, a small clump of thecal-interstitial tissue needs to be attached to the basement membrane. 4A), but within a day or two of culture, thecal cells from the very edge die, and a smooth, even layer of theca remains round the follicle.

And Day, B. N. (2000). Theriogenology, 54, 787. 33. Goff, A. , and Miron, P. (2001). Reprod. Domest. , 36, 19. 34. , and Cognie, Y. (2001). Theriogenology, 54, 209. 35. Ehrmann, R. , and Gey, G. O. (1956). J. Natl. , 16, 1375. 36. , and Mauchamp, J. (1981). J. , 91, 157. 37. , and Kato, S. (1994). J. Reprod. , 100, 333. 38. , Emery, B. , and Carrell, D. T. (2001). Biol. , 64, 375. 39. , Mobberley, M. , Moore, P. , Margara, R. , and Winston, R. M. (1997). Fertil. , 68, 682. 40. Telfer, E. , Binnie, J.

Best results from superovulation protocols are achieved by using prepubertal females (3–5 weeks old). Two hormones are administered by intraperitoneal injection 48 h apart. The first injection of pregnant mare’s serum gonadotrophin (PMSG) provides FSH that recruits follicles for ovulation. 2. 005g/l For protocols on how to prepare media, see instructions in chapter 3. 3. 001 followed 48 h later by an injection of human choronic gonadotrophin (hCG), which mimics the LH surge and results in ovulation.

Download PDF sample

A Laboratory Guide to the Mammalian Embryo by David K. Gardner, Michelle Lane, Andrew J. Watson


by William
4.2

Rated 4.52 of 5 – based on 25 votes